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ethidium bromide  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ethidium bromide
    Ethidium Bromide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethidium+bromide/Ethidium+bromide%2C+95%25/10__31926_slash_but__fwiafe__2026__19__68__1__11-39-126-129
    Average 97 stars, based on 1 article reviews
    ethidium bromide - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Staining:

    Article Title: Enhancing disease resilience in striped catfish (Pangasianodon hypophthalmus): an integrated assessment of environmental stressors, pathogen dynamics, and the protective efficacy of Spirulina platensis in Egyptian aquaculture.
    Article Snippet: The sustainable expansion of striped catfish (P. hypophthalmus) aquaculture in Egypt is constrained by environmental stressors, recurrent bacterial infections, and rising antimicrobial resistance.. This study offers the first integrated assessment of water quality parameters, pathogen identity and virulence, antimicrobial resistance (AMR) patterns, and the immunoprotective potential of dietary S. platensis under Egyptian farming conditions.. Field assessments from naturally infected farms revealed chronic hypoxia (mean DO 3.66 mg L−1) and persistent thermal stress (32.6 °C), both significantly outside recommended ranges, while nitrite levels approached the known toxicity threshold.

    Article Title: Bactericidal Mechanism of Chlorous Acid Water in the Inactivation of Non-Tuberculous Mycobacteria
    Article Snippet: To evaluate the amount of single-stranded DNA produced by treatment with CAW or NaClO, extracted bacterial DNAs were digested with S1 nuclease (Takara Bio, Kusatsu, Japan). .. The digested DNAs were electrophoresed in 0.8% Agarose S (Nippon Gene), and DNA fragmentation was evaluated using ethidium bromide (Thermo Fisher Scientifi Inc., Waltham, MA, USA) staining of the gels. .. After bactericidal assays were performed (treatment time 1 min) as described above, M. intracellulare NBRC 112750 cells were collected by centrifugation (14,000× g , 4 °C, 5 min), suspended in 1 mL of Middlebrook 7H9 medium supplemented with ADC (0.2% ( w / v ) dextrose, 0.2% ( v / v ) glycerol and 0.5% bovine serum albumin) and incubated at 37 °C for 30 min. As a control, we heated an M. intracellulare NBRC 112750 cell suspension at 100 °C for 10 min and filtered the suspension through a 40 μm cell strainer (Corning Inc., Corning, NY, USA).

    Article Title: Tracing Sticky Trails: The Historical Biogeography of Australia’s Glandular Goose-foots ( Dysphania , Chenopodioideae, Amaranthaceae)
    Article Snippet: Total DNA was extracted from 5-20 mg of dried leaf-material using the standard DNeasy Plant Mini Kit (QIAGEN, Venlo, Netherlands) following the manufacturer’s instructions and eluted in 100 μl of AE buffer (10 mM Tris, 0.5 mM EDTA, pH=9.0). .. DNA quality and quantity were assessed using a 1.0% agarose gel stained with ethidium bromide and the Invitrogen Qubit® 4 Fluorometer (Thermo Fisher Scientific), respectively. .. Library preparation was performed according to the standard NEBNext Ultra II FS Library Prep Kit protocol (New England Biolabs, Ipswich, MA, USA), with targeted inserts of approximately 300 bp and half-volume reactions.

    Article Title: Bioprospecting for alginate-lyase producing microorganisms for brown seaweed bioprocessing
    Article Snippet: .. Successful PCR products were visualised on a 1% (w/v) agarose gel stained with ethidium bromide (Thermo Fisher Sci., RSA) and then purified using a Zymoclean Gel recovery kit (Zymo Research Corporation, USA). ..

    Agarose Gel Electrophoresis:

    Article Title: Tracing Sticky Trails: The Historical Biogeography of Australia’s Glandular Goose-foots ( Dysphania , Chenopodioideae, Amaranthaceae)
    Article Snippet: Total DNA was extracted from 5-20 mg of dried leaf-material using the standard DNeasy Plant Mini Kit (QIAGEN, Venlo, Netherlands) following the manufacturer’s instructions and eluted in 100 μl of AE buffer (10 mM Tris, 0.5 mM EDTA, pH=9.0). .. DNA quality and quantity were assessed using a 1.0% agarose gel stained with ethidium bromide and the Invitrogen Qubit® 4 Fluorometer (Thermo Fisher Scientific), respectively. .. Library preparation was performed according to the standard NEBNext Ultra II FS Library Prep Kit protocol (New England Biolabs, Ipswich, MA, USA), with targeted inserts of approximately 300 bp and half-volume reactions.

    Article Title: Morphological and Its Molecular Characterisation of Pathogenic Moulds Isolated from Cashews (Anacardium occidentale L.), Togo
    Article Snippet: .. Camera (Infinix Hot mobile), Silica gel (VWR Chemicals), Self-stick adhesive envelope (Sotimex), plastic envelope (Domedia), Horizontal laminar flow hood (Air Science MultiplexTM), Refrigeratorfreezer (REX®), Petri dishes (DiaTrust Medical Co.,Ltd), Scalpel Handle (Swann Morton), Sodium hypochlorite 12° chlorine (Roi detergents), Ethanol 96° (ITW Reagents), Scalpel Chirurgical Lame (Swann Morton), Chloramphenicol (Zymo Research corporation), Potato Dextrose Agar (PDA, Thermo Fisher ScientificTM OxoidTM), Malt Extract Agar (MEA, Thermo Fisher ScientificTM OxoidTM), Microblue microscope (EUROMEX®), Microscope slides (MenzelGlaser), Coverslip (Deckglaser), LactoPhenol Cotton Blue (LPCB, Thermo Fisher ScientificTM), Potato Dextrose Broth (PDB, Thermo Fisher ScientificTM OxoidTM), Quick-DNATM Fungal/bacterial Miniprep Kit (Zymo Research corporation), SpectraMax® QuickDropTM (Molecular Devices®), Mini Personal Thermal Cycler (MultiGeneTM), Standard PCR reagents and Kits (Hhal, Hin1II(NIaIII), RsaI) (Thermo ScientificTM), Mini Gel Electrophoresis System, Model B2 | eBay (ThermoScientificTM), EThidium Bromide (BET, Thermo Fisher ScientificTM), Agarose gel (Thermo Fisher ScientificTM) were used. ..

    Article Title: Simple, rapid and cost-effective DNA extraction techniques for detection of economically important fruit flies in India
    Article Snippet: Each 25 μl of reaction mixture contained 12.5 μl of DreamTaq® Master Mix (Thermo Scientific), 1 μl of each primer (10 μM), 1 μl of template DNA and 9.5 μl of nuclease free water. .. The thermal cycling parameters, followed for reaction were as follows: initial denaturation at 95 °C for 4 min; 35 cycles of 95 °C for 1 min, 52 °C for 1 min, and 72 °C for 1 min 30 sec ; followed by a final extension at 72 °C for 7 min. Products of PCR reaction were segregated through gel electrophoresis in 1.6% agarose gel containing ethidium bromide (Thermo Scientific) and 100 bp DNA ladder (GeneDirex®). .. Results were visualised and documented using gel documentation unit (BioRad Laboratories Inc. USA).

    Article Title: SIRT6 overexpression counteracts chromatin aging in the male murine liver.
    Article Snippet: .. Concentration was determined using Nanodrop, and fragmentation was verified by running in an ethidium-bromide 1.5% agarose gel. ..

    Article Title: Bioprospecting for alginate-lyase producing microorganisms for brown seaweed bioprocessing
    Article Snippet: .. Successful PCR products were visualised on a 1% (w/v) agarose gel stained with ethidium bromide (Thermo Fisher Sci., RSA) and then purified using a Zymoclean Gel recovery kit (Zymo Research Corporation, USA). ..

    Adhesive:

    Article Title: Morphological and Its Molecular Characterisation of Pathogenic Moulds Isolated from Cashews (Anacardium occidentale L.), Togo
    Article Snippet: .. Camera (Infinix Hot mobile), Silica gel (VWR Chemicals), Self-stick adhesive envelope (Sotimex), plastic envelope (Domedia), Horizontal laminar flow hood (Air Science MultiplexTM), Refrigeratorfreezer (REX®), Petri dishes (DiaTrust Medical Co.,Ltd), Scalpel Handle (Swann Morton), Sodium hypochlorite 12° chlorine (Roi detergents), Ethanol 96° (ITW Reagents), Scalpel Chirurgical Lame (Swann Morton), Chloramphenicol (Zymo Research corporation), Potato Dextrose Agar (PDA, Thermo Fisher ScientificTM OxoidTM), Malt Extract Agar (MEA, Thermo Fisher ScientificTM OxoidTM), Microblue microscope (EUROMEX®), Microscope slides (MenzelGlaser), Coverslip (Deckglaser), LactoPhenol Cotton Blue (LPCB, Thermo Fisher ScientificTM), Potato Dextrose Broth (PDB, Thermo Fisher ScientificTM OxoidTM), Quick-DNATM Fungal/bacterial Miniprep Kit (Zymo Research corporation), SpectraMax® QuickDropTM (Molecular Devices®), Mini Personal Thermal Cycler (MultiGeneTM), Standard PCR reagents and Kits (Hhal, Hin1II(NIaIII), RsaI) (Thermo ScientificTM), Mini Gel Electrophoresis System, Model B2 | eBay (ThermoScientificTM), EThidium Bromide (BET, Thermo Fisher ScientificTM), Agarose gel (Thermo Fisher ScientificTM) were used. ..

    Microelectrode Array:

    Article Title: Morphological and Its Molecular Characterisation of Pathogenic Moulds Isolated from Cashews (Anacardium occidentale L.), Togo
    Article Snippet: .. Camera (Infinix Hot mobile), Silica gel (VWR Chemicals), Self-stick adhesive envelope (Sotimex), plastic envelope (Domedia), Horizontal laminar flow hood (Air Science MultiplexTM), Refrigeratorfreezer (REX®), Petri dishes (DiaTrust Medical Co.,Ltd), Scalpel Handle (Swann Morton), Sodium hypochlorite 12° chlorine (Roi detergents), Ethanol 96° (ITW Reagents), Scalpel Chirurgical Lame (Swann Morton), Chloramphenicol (Zymo Research corporation), Potato Dextrose Agar (PDA, Thermo Fisher ScientificTM OxoidTM), Malt Extract Agar (MEA, Thermo Fisher ScientificTM OxoidTM), Microblue microscope (EUROMEX®), Microscope slides (MenzelGlaser), Coverslip (Deckglaser), LactoPhenol Cotton Blue (LPCB, Thermo Fisher ScientificTM), Potato Dextrose Broth (PDB, Thermo Fisher ScientificTM OxoidTM), Quick-DNATM Fungal/bacterial Miniprep Kit (Zymo Research corporation), SpectraMax® QuickDropTM (Molecular Devices®), Mini Personal Thermal Cycler (MultiGeneTM), Standard PCR reagents and Kits (Hhal, Hin1II(NIaIII), RsaI) (Thermo ScientificTM), Mini Gel Electrophoresis System, Model B2 | eBay (ThermoScientificTM), EThidium Bromide (BET, Thermo Fisher ScientificTM), Agarose gel (Thermo Fisher ScientificTM) were used. ..

    Microscopy:

    Article Title: Morphological and Its Molecular Characterisation of Pathogenic Moulds Isolated from Cashews (Anacardium occidentale L.), Togo
    Article Snippet: .. Camera (Infinix Hot mobile), Silica gel (VWR Chemicals), Self-stick adhesive envelope (Sotimex), plastic envelope (Domedia), Horizontal laminar flow hood (Air Science MultiplexTM), Refrigeratorfreezer (REX®), Petri dishes (DiaTrust Medical Co.,Ltd), Scalpel Handle (Swann Morton), Sodium hypochlorite 12° chlorine (Roi detergents), Ethanol 96° (ITW Reagents), Scalpel Chirurgical Lame (Swann Morton), Chloramphenicol (Zymo Research corporation), Potato Dextrose Agar (PDA, Thermo Fisher ScientificTM OxoidTM), Malt Extract Agar (MEA, Thermo Fisher ScientificTM OxoidTM), Microblue microscope (EUROMEX®), Microscope slides (MenzelGlaser), Coverslip (Deckglaser), LactoPhenol Cotton Blue (LPCB, Thermo Fisher ScientificTM), Potato Dextrose Broth (PDB, Thermo Fisher ScientificTM OxoidTM), Quick-DNATM Fungal/bacterial Miniprep Kit (Zymo Research corporation), SpectraMax® QuickDropTM (Molecular Devices®), Mini Personal Thermal Cycler (MultiGeneTM), Standard PCR reagents and Kits (Hhal, Hin1II(NIaIII), RsaI) (Thermo ScientificTM), Mini Gel Electrophoresis System, Model B2 | eBay (ThermoScientificTM), EThidium Bromide (BET, Thermo Fisher ScientificTM), Agarose gel (Thermo Fisher ScientificTM) were used. ..

    Polymerase Chain Reaction:

    Article Title: Morphological and Its Molecular Characterisation of Pathogenic Moulds Isolated from Cashews (Anacardium occidentale L.), Togo
    Article Snippet: .. Camera (Infinix Hot mobile), Silica gel (VWR Chemicals), Self-stick adhesive envelope (Sotimex), plastic envelope (Domedia), Horizontal laminar flow hood (Air Science MultiplexTM), Refrigeratorfreezer (REX®), Petri dishes (DiaTrust Medical Co.,Ltd), Scalpel Handle (Swann Morton), Sodium hypochlorite 12° chlorine (Roi detergents), Ethanol 96° (ITW Reagents), Scalpel Chirurgical Lame (Swann Morton), Chloramphenicol (Zymo Research corporation), Potato Dextrose Agar (PDA, Thermo Fisher ScientificTM OxoidTM), Malt Extract Agar (MEA, Thermo Fisher ScientificTM OxoidTM), Microblue microscope (EUROMEX®), Microscope slides (MenzelGlaser), Coverslip (Deckglaser), LactoPhenol Cotton Blue (LPCB, Thermo Fisher ScientificTM), Potato Dextrose Broth (PDB, Thermo Fisher ScientificTM OxoidTM), Quick-DNATM Fungal/bacterial Miniprep Kit (Zymo Research corporation), SpectraMax® QuickDropTM (Molecular Devices®), Mini Personal Thermal Cycler (MultiGeneTM), Standard PCR reagents and Kits (Hhal, Hin1II(NIaIII), RsaI) (Thermo ScientificTM), Mini Gel Electrophoresis System, Model B2 | eBay (ThermoScientificTM), EThidium Bromide (BET, Thermo Fisher ScientificTM), Agarose gel (Thermo Fisher ScientificTM) were used. ..

    Article Title: Simple, rapid and cost-effective DNA extraction techniques for detection of economically important fruit flies in India
    Article Snippet: Each 25 μl of reaction mixture contained 12.5 μl of DreamTaq® Master Mix (Thermo Scientific), 1 μl of each primer (10 μM), 1 μl of template DNA and 9.5 μl of nuclease free water. .. The thermal cycling parameters, followed for reaction were as follows: initial denaturation at 95 °C for 4 min; 35 cycles of 95 °C for 1 min, 52 °C for 1 min, and 72 °C for 1 min 30 sec ; followed by a final extension at 72 °C for 7 min. Products of PCR reaction were segregated through gel electrophoresis in 1.6% agarose gel containing ethidium bromide (Thermo Scientific) and 100 bp DNA ladder (GeneDirex®). .. Results were visualised and documented using gel documentation unit (BioRad Laboratories Inc. USA).

    Article Title: Bioprospecting for alginate-lyase producing microorganisms for brown seaweed bioprocessing
    Article Snippet: .. Successful PCR products were visualised on a 1% (w/v) agarose gel stained with ethidium bromide (Thermo Fisher Sci., RSA) and then purified using a Zymoclean Gel recovery kit (Zymo Research Corporation, USA). ..

    Nucleic Acid Electrophoresis:

    Article Title: Morphological and Its Molecular Characterisation of Pathogenic Moulds Isolated from Cashews (Anacardium occidentale L.), Togo
    Article Snippet: .. Camera (Infinix Hot mobile), Silica gel (VWR Chemicals), Self-stick adhesive envelope (Sotimex), plastic envelope (Domedia), Horizontal laminar flow hood (Air Science MultiplexTM), Refrigeratorfreezer (REX®), Petri dishes (DiaTrust Medical Co.,Ltd), Scalpel Handle (Swann Morton), Sodium hypochlorite 12° chlorine (Roi detergents), Ethanol 96° (ITW Reagents), Scalpel Chirurgical Lame (Swann Morton), Chloramphenicol (Zymo Research corporation), Potato Dextrose Agar (PDA, Thermo Fisher ScientificTM OxoidTM), Malt Extract Agar (MEA, Thermo Fisher ScientificTM OxoidTM), Microblue microscope (EUROMEX®), Microscope slides (MenzelGlaser), Coverslip (Deckglaser), LactoPhenol Cotton Blue (LPCB, Thermo Fisher ScientificTM), Potato Dextrose Broth (PDB, Thermo Fisher ScientificTM OxoidTM), Quick-DNATM Fungal/bacterial Miniprep Kit (Zymo Research corporation), SpectraMax® QuickDropTM (Molecular Devices®), Mini Personal Thermal Cycler (MultiGeneTM), Standard PCR reagents and Kits (Hhal, Hin1II(NIaIII), RsaI) (Thermo ScientificTM), Mini Gel Electrophoresis System, Model B2 | eBay (ThermoScientificTM), EThidium Bromide (BET, Thermo Fisher ScientificTM), Agarose gel (Thermo Fisher ScientificTM) were used. ..

    Article Title: Simple, rapid and cost-effective DNA extraction techniques for detection of economically important fruit flies in India
    Article Snippet: Each 25 μl of reaction mixture contained 12.5 μl of DreamTaq® Master Mix (Thermo Scientific), 1 μl of each primer (10 μM), 1 μl of template DNA and 9.5 μl of nuclease free water. .. The thermal cycling parameters, followed for reaction were as follows: initial denaturation at 95 °C for 4 min; 35 cycles of 95 °C for 1 min, 52 °C for 1 min, and 72 °C for 1 min 30 sec ; followed by a final extension at 72 °C for 7 min. Products of PCR reaction were segregated through gel electrophoresis in 1.6% agarose gel containing ethidium bromide (Thermo Scientific) and 100 bp DNA ladder (GeneDirex®). .. Results were visualised and documented using gel documentation unit (BioRad Laboratories Inc. USA).

    Article Title: Susceptibility of the Placenta and Fetal Brain to Maternal Probiotic Supplementation
    Article Snippet: PCR analysis was done with the Green Master Mix (Promega, Madison, WI, USA) and according to the manufacturer’s recommendations. .. The reactions were run on 2% agarose (ThermoFisher Scientific, Waltham, MA, USA) gel containing ethidium bromide (ThermoFisher Scientific) with gel electrophoresis for one hour at 200 milliamps and 141 volts. ..

    Concentration Assay:

    Article Title: SIRT6 overexpression counteracts chromatin aging in the male murine liver.
    Article Snippet: .. Concentration was determined using Nanodrop, and fragmentation was verified by running in an ethidium-bromide 1.5% agarose gel. ..

    Purification:

    Article Title: Bioprospecting for alginate-lyase producing microorganisms for brown seaweed bioprocessing
    Article Snippet: .. Successful PCR products were visualised on a 1% (w/v) agarose gel stained with ethidium bromide (Thermo Fisher Sci., RSA) and then purified using a Zymoclean Gel recovery kit (Zymo Research Corporation, USA). ..



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